Visible control of the reaction  Up to 5 kb

DESCRIPTION
BioThermRed™ DNA polymerase is a mixture of BioTherm™ DNA polymerase and red pigment that works like BioTherm™. After addition of BioThermRed™ a thin red layer on the bottom of the tube appears. So you have a visible pipetting control. Furthermore you can see, if your reaction is already mixed, when the color of your reaction becomes uniform.

CONCENTRATION
5 units/µl

UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acidinsoluble form
in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxy-methyl)-methyl-amino-pro-panesulfonic
acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl₂, 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.

STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 0.2% indicator red dye, 50% glycerol (v/v). The 10x reaction buffer (on request with or without MgCl₂) is delivered free of charge.

STORAGE TEMPERATURE
Store BioThermRed™ DNA Polymerase below 0ºC, preferably at -20°C, in a constant temperature freezer.

10X REACTION BUFER
160 mM (NH₄)₂SO₄, 670 mM Tris-HCl pH 8.8 (at 25°C), 15 mM MgCl₂, 0.1% Tween 20

1.5 ml 10x reaction buffer (contains 15 mM MgCl₂) Cat. No. GC-002-006
1.5 ml 10x reaction buffer without MgCl₂ plus 50 mM MgCl₂ separately Cat. No. GC-002-007

ASSOCIATED ACTIVITIES
Endonuclease- and exonuclease activities were not detectable after 2 and 1 hours incubation, respectively, of
1 µg lambda DNA and 0.22 µg of EcoRI digested lambda DNA, respectively, at 72°C in the presence of 15-
20 units of BioThermRed™ DNA Polymerase.

PRODUCT CITATIONS
Bartsch, O.; Bartlick, B. & Ivell, R. (2004), J Clin Endocrinol Metab 89(1), 324--334.
Laimer, M.; Klausegger, A.; Aberer, W.; Oender, K.; Steinhuber, M.; Lanschuetzer, C. M.; Wally, V.; Hintner, H. & Bauer, J. W. (2006), Genet Med 8(4), 249--254