DESCRIPTION
SynergyPlatinum™ is a mix of thermostable polymerases  possessing 5'-3' DNA polymerase activity that is optimized to amplify longer GC-rich fragments (KlenThermN™) and 3'-5' proof-reading activity (AccuTherm™).These components are optimized to achieve amplification of 15 kb products from genomic DNA. A mixture of KlenThermN™ with AccuTherm™ provides more robust synthesis of longer GC-rich amplification products.

CONCENTRATION
10 units/µl

UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2, 1 mM ß-mercaptoethanol) and activated calf thymus DNA as substrate.

STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0,100 mM NaCI, 0.5 mM EDTA;1 mM DTT,0.01% Tween 20; 50% glycerol (v/v)

STORAGE TEMPERATURE
Store SynergyN™ DNA polymerase below 0°C,preferably at -20°C, in a constant temperature freezer.

10X REACTION BUFFER
160 mM (NH)₄SO₄, 670 mM Tris-HCl pH 9,1; 8 % Glycerol, 2 % DMSO,35 mM MgCl₂
Please note the difference between SynergyPlatinum™ and BioTherm™ reaction buffers.