DESCRIPTION
E. coli uracil-DNA glycosylase (UDG) is the recombinant form of the enzyme, which catalyzes the release of free uracil from uracil-containing DNA (U-DNA) and creates an alkali-sensitive apyrimidinic site in the DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded U-DNA, but not from oligomers (6 or fewer bases).

APPLICATION
PCR
RT-PCR
site-directed mutagenesis
as a probe for protein-DNA interaction studies producing highly labeled oligonucleotide probes

CONCENTRATION
5 units/µl

UNIT DEFINITION
One unit of enzyme activity is the amount that catalyzes the degradation of 1 µg single-stranded uracil-containing DNA at 37°C in 60 minutes.

STORAGE BUFFER
20 mM Tris-HCl pH 8.0; 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 0.1 mg/ml BSA; 50% glycerol

STORAGE TEMPERATURE
Storage temperature -20°C

10X REACTION BUFFER
500 mM Tricine pH 8.5, 10 mM MgCl₂ or buffer for PCR or RT-PCR protocol

QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.