DESCRIPTION
Isolated from recombinant E. coli strain this DNA-dependent RNA polymerase is strictly for its promoter. It is used to transcribe RNA from DNA templates, cloned into vectors containing T7 promoter.

APLICATION
 Radiolabeled RNA probe preparation
 RNA generation for in vitro translation
 RNA generation for studies of RNA structure, processing and catalysis
 Expression control via anti-sense RNA

CONCENTRATION
60 units/µl

UNIT DEFINITION
One unit is the amount of enzyme that incorporates 1 nmol of labelled nucleoside triphosphates into acid-precipitable RNA in 60 minutes at 37°C.

STORAGE BUFFER
20mM Tris-HCl pH 7.5, 100mM NaCl, 0,1mM EDTA, 2mM DTT, 0,1mg/ml BSA, 50% glycerol (v/v)

STORAGE TEMPERATURE
Store T7 RNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.

5X REACTION BUFFER
200mM Tris-HCl pH 7.9, 30mM MgCl₂, 50mM DTT