DESCRIPTION
Isolated from a recombinant E. coli strain this enzyme catalyzes the transfer of the terminal phosphate group of ATP to 5'-OH ends of DNA. T4 polynucleotide kinase is suited for labelling of 5'-OH ends of DNA with a ³²P-ATP.

APLICATION
End-labeling DNA or RNA for probes and DNA sequencing
Addition of 5´-phosphates to oligonucleotides to allow subsequent ligation
Removal of 3´-phosphoryl groups

CONCENTRATION
10 units/µl

UNIT DEFINITION
One unit is the amount of enzyme that incorporates 1 nmol of acid-insoluble ³²P in 30 minutes at 37ºC.

STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0; 100 mM NaCl; 0.5 mM EDTA; 1 mM DTT; 0.01% Tween 20; 50% glycerol (v/v)

STORAGE TEMPERATURE
Store T4 polynucleotide kinase below 0ºC, preferably at -20ºC, in a constant temperature freezer.

5X REACTION BUFFER
350 mM Tris-HCl pH 7.6; 50 mM MgCl₂; 25 mM DTT

PRODUCT CITATIONS
Hassan, M.; Feyen, O. & Grinstein, E. (2009), Cell Oncol 31(6), 437--456.
Selimovic, D.; Hassan, M.; Haikel, Y. & Hengge, U. R. (2008), Cell Signal 20(2), 311--322.