DESCRIPTION
Synergy mixture with addition of TthPlus DNA polymerase especially suited for low specificity PCR with degenerated primers.

APPLICATION
Low specificity PCR for degenerated primers

CONCENTRATION
10 units/µl

UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)methyl-amino-propanesulphonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl₂, 1 mM β-mercaptoethanol) and activated calf thymus DNA as substrate.

10X REACTION BUFER
160 mM (NH)₄SO₄, 670 mM Tris-HCl pH 9.1; 8% Glycerol, 2% DMSO, 35 mM MgCl₂
Please note the difference between Synergy™ and BioTherm™ reaction buffers.

STORAGE BUFFER
10 mM K-phospate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)

STORAGE TEMPERATURE
Store SynergyT™ DNA Polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.

QUALITY CONTROL
Activity, SDS-PAGE purity, absence of endonucleases/nichases and exonucleases.

REFERENCES
1. Barnes W.M. 1994. Proc. Natl. Acad. Sci. USA 91: 2216-2220

Figure 1. Electrophoresis in 1.5% agarose gel of PCR products obtained by detection of LTR in human locus 7p22.3 an higher primates. The presence of LTR in this locus was detected by a PCR product of 1831 bp using either Synergy or SynergyT. As templates were used DNA from human (1), chimpanzee (2), gorilla (3) orang-utan (4) and gibbon (5).