DESCRIPTION
Synergy is a mix of thermostable components possessing 5'-3' DNA polymerase activity (KlenTherm™) and a blend of enzymes (patent pending) improving the accuracy and processivity of PCR. These components are optimized to achieve amplification of 35 kb or 10 kb products from lambda templates or genomic DNA, respectively.

CONCENTRATION
10 units/µl

UNIT DEFINITION
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl₂, 1 mM β-mercaptoethanol) and activated calf thymus DNA as substrate.

STORAGE BUFFER
10 mM K-phosphate buffer pH 7.0; 100 mM NaCI; 0,5 mM EDTA; 1 mM DTT; 0.01% Tween 20; 50% glycerol (v/v)

STORAGE TEMPERATURE
Store Synergy™ DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.

10X REACTION BUFER
160 mM (NH)₄SO₄, 670 mM Tris-HCl pH 9.1; 8% Glycerol, 2% DMSO, 35 mM MgCl₂
Please note the difference between Synergy™ and BioTherm™ reaction buffers.

PRODUCT CITATIONS
Klimacek, M.; Hellmer, H. & Nidetzky, B. (2007), Biochem J 404(3), 421--429.
Tittarelli, A.; Milla, L.; Vargas, F.; Morales, A.; Neupert, C.; Meisel, L. A.; Salvo-G, H.; Peñaloza, E.; Muñoz, G.; Corcuera, L. J. & Silva, H. (2007), J Exp Bot 58(10), 2573--2582.
Tittarelli, A.; Santiago, M.; Morales, A.; Meisel, L. A. & Silva, H. (2009), BMC Plant Biol 9, 121.