Isolated from recombinant E. coli strain this DNA-dependent RNA polymerase is strictly for its promoter. It is used to transcribe RNA from DNA templates, cloned into vectors containing T7 promoter.
One unit is the amount of enzyme that incorporates 1 nmol of labelled nucleoside triphosphates into acid-precipitable RNA in 60 minutes at 37°C.
20mM Tris-HCl pH 7,5, 100mM NaCl, 0,1mM EDTA, 2mM DTT, 0,1mg/ml BSA, 50% glycerol (v/v)
Store T7 RNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer or near by North Pole.
5X REACTION BUFFER
200mM Tris-HCl pH 7,9, 30mM MgCl2, 50mM DTT