SynergyT™ DNA Polymerase

Produkt Artikel-Nr. Packungsgröße Preise
SynergyT™ DNA Polymerase
GC-049-0100
Verfügbarkeit:
100 u
30,00 € *
SynergyT™ DNA Polymerase
GC-049-0250
Verfügbarkeit:
250 u
75,00 € *
SynergyT™ DNA Polymerase
GC-049-0500
Verfügbarkeit:
500 u
150,00 € *
SynergyT™ DNA Polymerase
GC-049-1000
Verfügbarkeit:
1000 u
300,00 € *
SynergyT™ DNA Polymerase
GC-049-5000
Verfügbarkeit:
5000 u
1.500,00 € *

DESCRIPTION

Synergy mixture with addition of TthPlus DNA polymerase especially suited for low specificity PCR with degenerated primers.


APPLICATION

  • Low specificity PCR for degenerated primers


CONCENTRATION

10 units/µl


UNIT DEFINITION

One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)methyl-amino-propanesulphonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2 , 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.


10X REACTION BUFER

160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2

Please note the difference between Synergy™ and BioTherm™ reaction buffers.


STORAGE BUFFER

10 mM K-phospate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20, 50% glycerol (v/v)


STORAGE TEMPERATURE

Store SynergyT™ DNA Polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.


QUALITY CONTROL

Activity, SDS-PAGE purity, absence of endonucleases/nichases and exonucleases.


REFERENCES

  1. Barnes W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from bacterio-phage templates. Proc. Natl. Acad. Sci. USA 91: 2216-2220


COMPARISON OF Synergy AND SynergyT UNDER SPECIAL REACTION CONDITIONS