KlenTherm-based mixture with addition of pyrophosphatase for the amplification of long fragments. SynergyPlus™ DNA polymerase can amplify up to 20 kb from genomic DNA.
- Very Long and accurate PCR
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)methyl-amino-propanesulphonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2 , 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.
10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v)
Store SynergyPlus™ DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.
160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2
Please note the difference between Synergy™ and BioTherm™ reaction buffers.
Activity, SDS-PAGE purity, absence of endonucleases/ nickases and exonucleases.
Barnes W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from bacterio-phage templates. Proc. Natl. Acad. Sci. USA 91: 2216-2220